Lab Assay Development for Immune Monitoring in Organ Transplantation

Location: Melbourne, VIC

Duration: 3 months

Project Background

Tissue Typing Laboratories are responsible for defining the immune system of donors and recipient of organ transplant throughout Australia. There are five semi-independent labs that perform testing on a 24/7 basis in order to achieve optimal patient outcomes. These labs are constantly innovating and assessing new lab technique to maintain these desired outcomes.

Research to be Conducted

Before a tissue typing laboratory can implement a new technique it needs to be rigorously evaluated and validated to adhere to international and national laboratory standards. The student will be involved in these processes and be responsible for the initial evaluation of these new techniques which will be sequence and flow cytometry based. As part of these processes the student will not only be expected to perform these assay validations but will be tutored in the interpretation of the results and also guided through the quality assurance programmes that allow such assays to become mainstream testing going forward.

Skills Required

If you’re a PhD student and meet some or all the below we want to hear from you. We strongly encourage women, indigenous and disadvantaged candidates to apply:

  • Flow cytometer and fluorescent monoclonal antibody use – preferably on the BD Canto platform
  • Next generation sequencing experience preferably of the Illumina platform
  • Knowledge of Human Leukocyte Antigen (HLA) system
  • Understanding of laboratory test accuracy and reproducibility

Expected Outcomes

The student will become proficient in the use of flow cytometry and next generation sequencing in the context of delivering of clinically validated result that adheres to stringent national and international standards. The student will also acquired the knowledge base to interpret highly complex results from PCR, sequencing and flow cytometry data. The 3 month placement will also allow the student understand the complexity and work flows in an acute time pressured setting of organ transplantation in Australia.

Additional Details

The intern will receive $3,000 per month of the internship, usually in the form of stipend payments.

It is expected that the intern will primarily undertake this research project during regular business hours, spending at least 80% of their time on-site with the industry partner.  The intern will be expected to maintain contact with their academic mentor throughout the internship either through face-to-face or phone meetings as appropriate.

The intern and their academic mentor will have the opportunity to negotiate the project’s scope, milestones and timeline during the project planning stage.

Applications Close

27 November 2019

Reference

APR – 1169